TY -非盟的盖尔,瑞秋E盟——Kotnik杰克亨利AU -里昂,维多利亚盟——Brandstetter伊丽莎白盟——业主Suchsland,莫妮卡AU -汉,彼得•D AU -格雷厄姆Chelsey盟——Ilcisin Misja AU -金,阿什利·E盟——楚,海伦Y盟,Nickerson Deborah AU - Starita Lea M AU -贝德福德,特雷弗AU -鲁兹,巴里AU -汤普森,马修·J PY - 2022 DA - 2022/2/22 TI -家庭的诊断准确性,流感快速自检:前瞻性比较精度研究乔- JMIR公共卫生Surveill SP - e28268六世- 8 - 2 KW -流感KW -快速测试KW -急性呼吸道疾病KW - self-collection KW -自我KW - mHealth KW -移动健康KW -收集KW -家测试KW -手机AB -背景:快速诊断测试(RDTs)流感在家用个人可能扩大测试和减少流感对卫生系统的影响。改善获得检测的机会可能导致在症状出现后更早诊断，从而能够对检测呈阳性的人进行更快速的干预，包括改变行为以尽量减少传播。然而，在自我检测人群中，流感随机对照试验的准确性尚未确定。目的:本研究旨在评估急性呼吸道疾病非专业用户在家中进行流感RDT的准确性，并将同一个人邮寄到实验室进行参考检测的自采样本进行比较。方法:我们对有急性呼吸道疾病症状的个体进行了家庭流感RDT (Ellume)的比较准确性研究。参与者于2020年2月和3月从美国华盛顿的大西雅图地区招募。参与者邮寄流感RDT和参考样本收集材料，完成后返回中心实验室进行定量逆转录聚合酶链反应流感测试。我们探讨了年龄、流感类型、病程和症状严重程度对RDT准确性以及流感病毒和核糖核酸酶P(人类DNA标记物)周期阈值的影响。结果:共有605名参与者完成了所有研究步骤并被纳入我们的分析，其中87名(14.4%)通过定量逆转录聚合酶链反应检测出流感阳性(甲型流感70/ 87,80%，乙型流感17/ 87,20%)。与参考测试相比，RDT的总体敏感性和特异性分别为61% (95% CI 50%-71%)和95% (95% CI 93%-97%)。 Among individuals with symptom onset ≤72 hours, sensitivity was 63% (95% CI 48%-76%) and specificity was 94% (95% CI 91%-97%), whereas, for those with duration >72 hours, sensitivity and specificity were 58% (95% CI 41%-74%) and 96% (95% CI 93%-98%), respectively. Viral load on reference swabs was negatively correlated with symptom onset, and quantities of the endogenous marker gene ribonuclease P did not differ among reference standard positive and negative groups, age groups, or influenza subtypes. The RDT did not have higher sensitivity or specificity among those who reported more severe illnesses. Conclusions: The sensitivity and specificity of the self-test were comparable with those of influenza RDTs used in clinical settings. False-negative self-test results were more common when the test was used after 72 hours of symptom onset but were not related to inadequate swab collection or severity of illness. Therefore, the deployment of home tests may provide a valuable tool to support the management of influenza and other respiratory infections. SN - 2369-2960 UR - https://publichealth.www.mybigtv.com/2022/2/e28268 UR - https://doi.org/10.2196/28268 UR - http://www.ncbi.nlm.nih.gov/pubmed/35191852 DO - 10.2196/28268 ID - info:doi/10.2196/28268 ER -