TY - JOUR AU - Gabashvili, Irene S PY - 2020 DA - 20/11/4 TI -肠道微生物组中的皮肤细菌作为系统性恶臭和人对我过敏(PATM)条件的生物标志物:见解几乎进行临床试验的乔- JMIR北京医学SP - e10508六世- 3 - 1 KW -微生物KW -特发性体味KW -系统性恶臭KW - PATM KW - TMAU KW -湿润烧伤膏KW -腋臭KW -口臭KW -躯体变形障碍KW -虚拟保健KW -分散的临床试验KW -诊断KW -管理KW - patient-reported结果KW - PRO KW -家庭测试AB -背景:皮肤是一个动态的微生物生态系统，也是许多影响人体健康的化合物的来源。皮肤-微生物群的相互作用会导致持久的、具有心理社会破坏性的体味，尽管卫生状况良好。由于气味的产生往往是短暂的，在医学检查中可能察觉不到恶臭。因此，有气味的抱怨可以被诊断为身体畸形障碍，并进行心理评估。开发简单的家庭测试和虚拟护理程序可以改善对使社会衰弱的恶臭状况的诊断和管理。目的:本研究的目的是评估家庭肠道微生物组检测在诊断和治疗特发性身体和呼吸异味以及对我过敏综合征(PATM)患者中的潜在有效性。方法:我们通过电子邮件或社交媒体联系既往代谢体臭(MEBO)和PATM研究的参与者以及在线支持小组。同意参与的个人邮寄了用于在家收集肠道微生物组样本的测试包。 Participants completed an online survey (specially developed for this study) addressing their symptoms and other quality-of-life indicators at baseline and after sampling. Participants collected stool samples after flare-ups or symptom improvements and mailed them to the laboratory to be processed and analyzed. We evaluated between-group differences in symptom severity, as well as symptom improvement observations for the same individuals. For differential abundance testing of microbial taxa, we performed nonparametric statistical analyses using Mann-Whitney U tests for unpaired samples and Wilcoxon signed rank test for paired samples. Results: A total of 112 individuals from 21 countries consented to participate. About half the participants had been tested for the metabolic disorder trimethylaminuria, and about half of those tested were diagnosed with the disorder. The levels of bacteria previously associated with cutaneous body odor were significantly elevated in gut samples. For the combination of species from Anaerococcus, Corynebacterium, Campylobacter, and Propionibacterium genera, the differences were P=.002 for active (73 participants, 182 samples) versus regression or remission groups (30 participants, 51 samples); P=.01 for those experiencing symptoms most or all of the time (46 participants, 88 samples) versus those who had symptoms sometimes, rarely, or never (25 participants, 74 samples); and P<.001 for improvement of symptoms in the same individuals (22 participants, 43 sets of matched samples). Changes in microbial diversity were significant for between- but not within-participant comparisons. Conclusions: Changes in the gut microbiome composition affect MEBO and PATM severity. In particular, an increase in intestinal bacteria producing odor when in skin flexures was associated with increased intensity of self-reported symptoms. The changes were consistent in the within-group and between-group analyses. Our findings support the feasibility of remote and decentralized clinical studies of malodor conditions. Supplementary sample collection procedures may help to meet established research quality standards. Trial Registration: ClinicalTrials.gov NCT03582826; http://clinicaltrials.gov/ct2/show/NCT03582826 International Registered Report Identifier (IRRID): RR2-10.1101/2020.08.21.20179242 SN - 2562-0959 UR - http://derma.www.mybigtv.com/2020/1/e10508/ UR - https://doi.org/10.2196/10508 DO - 10.2196/10508 ID - info:doi/10.2196/10508 ER -